See the structural impact of a mutation before designing your primers.
PCR site-directed mutagenesis is one of the most often-used techniques for introducing a mutation into a DNA sequence, however most software tools don’t consider the impact of a mutation on protein structure when designing primers. Lasergene provides a unique approach to primer design for PCR site-directed mutagenesis by first focusing on predicting which mutations introduced into a DNA sequence may lead to changes in protein stability. Using our hot spot scanning and create variant tools, you can quickly see what mutations will increase or decrease fold stability. Once optimal candidate variants for your experiment are determined, you are ready to create PCR primers to introduce them into your sequence using our point-and-click tools for designing and mutating primers. The unique combination of our structure modeling and primer design tools saves you time and money compared to traditional trial-and-error approaches to mutagenesis experiments.