Import primers

To import primers from an external primer catalog:

1. Select File > Import Primers from a Catalog.

2. Choose a primer catalog (.pri).

SeqBuilder Pro attempts to locate each primer in the catalog on the current sequence by searching for sites on the template that match at least the 12-mer at the 3’ end of the primer. Those that are found are annotated as PCR_primer features and added to the Primers view. If primers were located, a message confirming the new primers will appear:

Each PCR_primer feature added as a result of importing primers will have three qualifiers:

• • /primer - the primer sequence
    
    
  • /note - any notes for the primer that were contained in the catalog
    
    
  • /set - the set number SeqBuilder Pro assigns to the group of imported primers. The Primers view uses set numbers to organize primers into groups.

*Note: After designing primers, a feature of the amplified segment is created. When you import the primers from a primer catalog, however, the amplification features are lost.This is by design, as primers can be imported onto an unrelated sequence. However, if you import primers from a catalog, the PCR_primer features appear in both the Features and Primers views. If you then select a pair of primers from the Primers view and choose Priming > Pair Selected Primers, a new PCR_product feature will be added to the Features view